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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Viegas , Susana; Oliveira, de Ana Cebola; Carolino, Elisabete; Pádua, Mário;

    Healthcare workers who prepare or administer cytotoxic agents run the risk of exposure, and the risks for health are real even at doses lower than those applied in cancer patients, because, in theory, no dose is safe. The most common and problematic route of exposure is through the skin, especially as work surfaces can remain contaminated even after cleaning. This pilot study aimed to demonstrate the importance of having an effective surface decontamination protocol by determining surface contamination with cyclophosphamide, 5-fluorouracil, and paclitaxel as the most common cytotoxic drugs in an oncology day service. Samples were collected before and after drug handling and analysed with high performance liquid chromatography with diode array detection (HPLC-DAD). Of the 29 samples collected before drug handling 23 were contaminated, five of which with more than one drug. Of the 30 samples collected after drug handling 25 were contaminated, eight of which with more than one drug. The two time points did not significantly differ, which evidences a widespread contamination and ineffective cleaning. This calls for revising the cleaning protocol and handling procedure to place contamination under control as much as possible. publishersversion published

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    Authors: Raquel Cruz-Duarte; Cátia Rebelo de Almeida; Magda Negrão; Afonso Fernandes; +18 Authors

    Purpose: Cetuximab is an EGFR-targeted therapy approved for the treatment of RAS wild-type (WT) metastatic colorectal cancer (mCRC). However, about 60% of these patients show innate resistance to cetuximab. To increase cetuximab efficacy, it is crucial to successfully identify responder patients, as well as to develop new therapeutic approaches to overcome cetuximab resistance. Experimental design: We evaluated the value of EGFR effector phospholipase C gamma 1 (PLCγ1) in predicting cetuximab responses, by analyzing progression-free survival (PFS) of a multicentric retrospective cohort of 94 treated patients with mCRC (log-rank test and Cox regression model). Furthermore, we used in vitro and zebrafish xenotransplant models to identify and target the mechanism behind PLCγ1-mediated resistance to cetuximab. Results: In this study, levels of PLCγ1 were found increased in RAS WT tumors and were able to predict cetuximab responses in clinical samples and in vitro and in vivo models. Mechanistically, PLCγ1 expression was found to bypass cetuximab-dependent EGFR inhibition by activating ERK and AKT pathways. This novel resistance mechanism involves a noncatalytic role of PLCγ1 SH2 tandem domains in the propagation of downstream signaling via SH2-containing protein tyrosine phosphatase 2 (SHP2). Accordingly, SHP2 inhibition sensitizes PLCγ1-resistant cells to cetuximab. Conclusions: Our discoveries reveal the potential of PLCγ1 as a predictive biomarker for cetuximab responses and suggest an alternative therapeutic approach to circumvent PLCγ1-mediated resistance to cetuximab in patients with RAS WT mCRC. In this way, this work contributes to the development of novel strategies in the medical management and treatment of patients with mCRC. M. Martins' research was supported by Liga Portuguesa Contra o Cancro (LPCC): Terry Fox Fundation; Investigador FCT- Fundação para a Ciência e Technologia (IF/00409/2014) and IMM Bridge grant; RC-D research was supported by Fundação para a Ciência e Technologia (SFRH/BD/139138/2018). A. Fernandes was supported by LPCC-IMM BIOBANK; R. Fior was supported by Champalimaud Foundation and L. Costa was supported by Merck Serono. © 2022 The Authors; Published by the American Association for Cancer Research. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 International (CC BY-NC-ND)

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    Clinical Cancer Research
    Article . 2022 . Peer-reviewed
    License: CC BY NC ND
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    Universidade de Lisboa: Repositório.UL
    Other literature type . 2022
    License: CC BY NC ND
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    Clinical Cancer Research
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      Clinical Cancer Research
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      Universidade de Lisboa: Repositório.UL
      Other literature type . 2022
      License: CC BY NC ND
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Clinical Cancer Research
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    Authors: Steidle, Bianca;

    Today’s consumers want brands to engage in socio political topics. However, this can be offensive and cause personal conflict. This work hypothesizes that when consumers think about these conflicts, their evaluation, and intentions towards brands that take a stand differ. Additionally, it examines how consumers’ reactions to a brand’s stand on a controversial vs. non-controversial topic influence conflict, attitudes, and purchase intention. Two main experiments were conducted. The results didn’t show a difference when consumers either did or didn’t think about conflict. Nonetheless, the controversial brand’s engagement increased conflict perception. This negatively impacted attitudes that were positively linked to purchase intentions.

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    Authors: Alves, Daniel;

    As freguesias de Lisboa sofreram profundas alterações ao longo dos séculos XIX e XX. Não só foram alteradas em número, mas também na sua dimensão geográfica. A última grande reforma, datada de 1959, modificou de tal forma os contornos das freguesias lisboetas que inviabiliza a utilização dos contornos actuais para a realização de estudos no passado. Procurando dar resposta a esta problemática e com base em cartografia histórica, foram desenhadas as freguesias em três períodos (1826, 1852 e 1909) sendo incluídas num sistema de informação geográfica, o que possibilita não só a análise da evolução administrativa da capital, mas também o estudo de muitas variáveis históricas, de base paroquial, para todo o século XIX e início do século XX.

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    Authors: Catry, F. X.; Rego, F. C.; Moreira, F.; Bação, Fernando;

    Catry, F. X., Rego, F. C., Moreira, F., & Bação, F. (2008). Characterizing and modelling the spatial patterns of wildfire ignitions in Portugal: Fire initiation and resulting burned area. In Modelling, Monitoring and Management of Forest Fires (Vol. 119, pp. 213-221). WITPress. https://doi.org/10.2495/FIVA080221 According to the statistics Portugal has the highest density of wildfire ignitions among southern European countries. The ability to predict ignition occurrence constitutes an important tool for managers, helping to improve the effectiveness of fire prevention, detection and fire fighting resources allocation. In this study we used a database with information about 127 490 fire ignitions that occurred in Portugal during a five year period. We performed frequency analysis to characterize the occurrence of wildfire ignitions in relation to both human and environmental variables and compared the spatial patterns of ignitions which originated fires larger or smaller than 500 ha. We also used logistic regression models to predict the relative probability of ignition occurrence, as a function of the resulting fire size. Results show that fire ignitions are strongly related to human presence and activity, and that the spatial patterns of ignitions are different for larger or smaller wildfires. Larger wildfires started in areas with lower population density, more distant from the main roads and at higher elevations, when compared to smaller fires, and also started more frequently in shrublands and forested areas. The results obtained can be useful in decision making for fire danger management. publishersversion published

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    Authors: De Simone, Mario; Alvigini, Laura; Alonso-Cotchico, Lur; Brissos, Vânia; +6 Authors

    Funding Information: This project has received funding from the Biobased Industries Joint Undertaking (JU) under Grant Agreement 837890 (SMARTBOX) and from Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) Grant 824017 (B-Ligzymes). The JU receives support from the European Union’s Horizon 2020 research and innovation program and the Biobased Industries Consortium (BIC). Fundação para a Ciência e a Tecnologia (FCT) additionally supported this work through R&D Unit MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020), LS4FUTURE Associated Laboratory (LA/P/0087/2020), and Project UID/Multi/04326/2019, from the operational programs CRESC Algarve 2020 and COMPETE for 2020 through Project EMBRC.PT ALG-01-0145-FEDER-022121. M.D.S. acknowledges a Ph.D. grant (2020.08246.BD) from FCT. Publisher Copyright: © 2022 American Chemical Society. Biocatalysis is a key tool in both green chemistry and biorefinery fields. NOV1 is a dioxygenase that catalyzes the one-step, coenzyme-free oxidation of isoeugenol into vanillin and holds enormous biotechnological potential for the complete valorization of lignin as a sustainable starting material for biobased chemicals, polymers, and materials. This study integrates computational, kinetic, structural, and biophysical approaches to characterize a new NOV1 variant featuring improved activity and stability compared to those of the wild type. The S283F replacement results in a 2-fold increased turnover rate (kcat) for isoeugenol and a 4-fold higher catalytic efficiency (kcat/Km) for molecular oxygen compared to those of the wild type. Furthermore, the variant exhibits a half-life that is 20-fold higher than that of the wild type, which most likely relates to the enhanced stabilization of the iron cofactor in the active site. Molecular dynamics supports this view, revealing that the S283F replacement decreases the optimal pKa and favors conformations of the iron-coordinating histidines compatible with an increased level of binding to iron. Importantly, whole cells containing the S283F variant catalyze the conversion of ≤100 mM isoeugenol to vanillin, yielding >99% molar conversion yields within 24 h. This integrative strategy provided a new enzyme for biotechnological applications and mechanistic insights that will facilitate the future design of robust and efficient biocatalysts. publishersversion published

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    Biochemistry
    Other literature type . Article . 2022 . Peer-reviewed
    License: CC BY NC ND
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    ZENODO
    Article . 2022
    License: CC BY
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    ZENODO
    Article . 2022
    License: CC BY
    Data sources: Datacite
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    Biochemistry
    Article . 2022
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      Biochemistry
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      ZENODO
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      Biochemistry
      Article . 2022
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    Authors: Pereira Coutinho, Francisco; Pereira dos Santos, Ruth Maria; Passos Silva, Matheus;

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    Authors: Chaquisse, Marta Francisco Mabote;

    Dissertação apresentada como requisito parcial para obtenção do grau de Mestre em Estatística e Gestão de Informação O presente trabalho, apresenta os resultados da análise da metodologia utilizada pelo Instituto Nacional de Estatística (INE) para avaliar a satisfação dos utilizadores da informação estatística oficial. A informação para a análise foi obtida através de uma entrevista aos funcionários do INE que participam directamente no processo da realização do inquérito de opinião sobre a satisfação dos utilizadores da informação estatística oficial e através da análise do questionário utilizado pelo INE no mesmo inquérito. Da análise feita conclui-se que a metodologia adoptada pelo INE para avaliação do grau de satisfação do utilizador é de natureza tradicional, baseada na descrição das respostas obtidas num inquérito aos utilizadores da informação estatística. Sugere-se como proposta de melhoria a utilização de uma abordagem estrutural para avaliação do grau de satisfação. Na abordagem estrutural, os indicadores de satisfação são obtidos através da estimação de um modelo de satisfação do cliente e não derivados directamente dos dados dos inquéritos aos utilizadores. É a estimação do modelo que fornece os índices, os impactos e os pesos que os indicadores associados às variáveis latentes contribuem na satisfação. Com base na abordagem sugerida foi desenvolvido e testado o modelo para avaliação da satisfação dos utilizadores da informação estatística produzida pelo INE de Moçambique, através do lançamento de um inquérito piloto aos utilizadores da informação estatística. Os resultados mostram que cerca de 51% da variação total da variável satisfação e 36% da variação total da variável confiança é explicada pelo modelo o que lhe confere uma razoável capacidade explicativa. A proporção da variância dos indicadores de medida explicada pela variável latente associada em todos os constructos (AVE) é aproximado ou superior a 0.5, podendo se concluir que o instrumento de medida tem validade convergente, ou seja, os itens utilizados medem o mesmo constructo em cada bloco. Os coeficientes de impacto das variáveis latentes estimados no modelo proposto, são positivos e estatisticamente significativos a um nível de confiança de 95%, com a excepcão da variável Qualidade da Informação Estatística.O índice médio da satisfação do utilizador da informação estatística estimado é de 7.0 numa escala de 1 a 10 pontos.

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    Authors: Madeira, Ana Maria Lavadinho; Teixeira, Joana; Botelho,  Fernanda; Costa, João; +4 Authors

    UID/LIN/03213/2013 PEst-OE/LIN/UI3213/2014 Este documento foi elaborado no âmbito do “Estudo de avaliação do impacto da aplicação do Português Língua Não Materna no sistema educativo português e definição de medidas prospetivas para a oferta desta área curricular nos Ensinos Básico e Secundário”. publishersversion published

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    Authors: dos Santos, Mariana de Lemos Pinto;

    UID/PAM/00417/2019 PTDC/ART-HIS/29837/2017 É reconhecido por vários autores o carácter visual da escrita do artista português Almada Negreiros (1893-1970), mas as múltiplas derivações e interpretações que daí podem surgir têm ainda muito por explorar. Neste artigo procuro fazer algumas dessas interpretações sobre o intercâmbio entre o pictórico e o literário que ocorre na obra de Almada Negreiros, com ênfase precisamente no intercâmbio, isto é, não notando meramente a criação de imagens visuais literárias — o que tem sido o foco dos estudos literários — mas entendendo que a escrita de Almada deriva de uma concepção de vanguarda e de arte assente no primado da visão e que a sua prática e teoria pictóricas determinam a sua escrita. Por outro lado, abordarei também a revisitação que faz em pintura da sua obra literária. Mais do que um diálogo entre duas artes, o pictórico e o literário em Almada estão profundamente interligados e a obra daqui resultante constitui-se um exemplo da inventividade que resultou da ruptura de fronteiras entre as artes operada pelas vanguardas do início do século. Many authors have recognized the visual character of the writings by the Portuguese artist Almada Negreiros (1893-1970), but the multiple variations and interpretations that can be driven from that are yet to be explored. In this article I intend to make some of those interpretations about the interchange between the pictorial and the literary that occur in his work, emphasizing precisely the interchange, that is, not noticing merely how he creates strong literary images — which has been the focus of the literary studies — but understanding that Almada’s writing thrives from a notion of avant-garde based on the primacy of vision and that his practice and theory about the pictorial are determinant for his writing. On another hand, I will also consider how his painting often revisits his literary work. More than a dialogue between two art forms, the pictorial and the literary in Almada Negreiros are profoundly interconnected and constitute an example of the inventiveness that resulted from the rupture of the frontiers between the arts operated by the avant-garde from the early twentieth century. publishersversion published

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    Authors: Benavente, Ana Luísa da Silva Ribeiro;

    Mucinous carcinomas are tumors with poor prognosis and secrete a large amount of mucus. The development of an in vitro model that reconstitutes the mucin-rich microenvironment of mucinous carci- nomas can provide a better understanding on how the aberrant mucus production relates to the pro- gression of these tumors. Furthermore, it responds to the lack of predictive in vitro models and leads the way to the replacement of standard animal models. In this project, colorectal mucinous carcinomas are studied, for which several mucin-secreting cell lines are available. We demonstrate that crosslinked bovine submaxillary mucin gels function as a platform to encapsulate cancer cells and obtain spheroids that grow and proliferate over 10 days. More importantly, they resist 5-FU and the drug resistance was different between cell lines. Although at a slower rate than HT-29 MTX, LS174T cells reached equivalent spheroids diameter (156.5 and 187.9 μm, respectively) and metabolic activity (3- and 4- fold increases, respectively). LS174T also showed the greater physical barrier effect against 5-FU in monolayer (2D) or encapsulated (3D) in BSM, but also a potential biological barrier caused by cell entanglement in mucin-gel for 10 days. In the 2D model, LS174T cell viability went from 45.9 ± 8.81% without gel to 84.2 ± 1.96% with muc-gel covering the cells. When encapsulated and further challenged with 50.0 mM of 5-FU, 59.6 ± 7.56% cells were viable after 6 h, whereas 76.2 ± 5.20% of cells survived after 10 days of interaction with BSM. Thus, these results unlock an exciting path to apply mucins as a scaffold for mucinous cancer cells and enlighten necessary refinements to be implemented in future studies towards the ultimate goal of building a robust 3D model capable of recreating the in vivo tumor microenvironment and serving as a high-throughput platform for drug screening in the pharmaceutical industry. Os carcinomas mucinosos são tumores com uma reduzida oportunidade de recuperação e secretam uma grande quantidade de muco. O desenvolvimento de um modelo in vitro que reconstitua o microambiente rico em mucinas de um cancro mucinoso pode proporcionar conhecimentos pertinen- tes acerca da relação entre a produção irregular de muco e a progressão deste tipo de tumores. Além disso, preenche a lacuna de modelos in vitro disponíveis e abre horizontes para a substituição de mo- delos standard de animais. Este projeto focou-se no cancro colorectal mucinoso, para o qual existem várias linhas celulares secretoras de muco. Demonstra-se que o hidrogel de mucinas funciona para a encapsulação de células de cancro e obtenção de esferóides que crescem e proliferam durante 10 dias. Principalmente, demon- stra-se resistência a 5-FU, que diferiu entre linhas celulares. Embora mais lentamente do que as células HT-29 MTX, os esferóides de LS174T atingiram diâmetro e atividade metabólica semelhantes. LS174T revelou um maior efeito de barreira física contra o fármaco 5-FU nos modelos 2D e 3D. Neste último, os resultados sugeriram um potencial efeito biológico resultante da interação das células com BSM durante 10 dias. Constatou-se, no modelo 2D, uma maior viabilidade celular de LS174T na presença de BSM (84.2 ± 1.96%) comparando com a ausência de hidrogel (45.9 ± 8.81%). Relativamente à encapsulação e ensaio com 50.0 mM de 5-FU, 59.6 ± 7.56% das células eram viáveis após 6 h, en- quanto após 10 dias de interação com BSM verificou-se 76.2 ± 5.20% de viabilidade. Estes resultados inauguram um percurso entusiasmante para a aplicação de mucinas como uma platafoma para células de cancros mucinosos e elucidam acerca de melhorias necessárias em estudos futuros, com um objetivo maior: o desenvolvimento de um modelo 3D robusto, capaz de recrear o mi- croambiente in vivo tumoral e de ser uma plataforma de alto rendimento para drug screening na in- dústria farmacêutica.

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    Authors: Amaral, Maria Odete Pereira;

    RESUMO - Introdução: Os problemas do sono, designadamente a insónia, os sintomas de insónia, os padrões de sono inadequados e a sonolência diurna, são frequentes na adolescência. Estes problemas estão frequentemente associados a múltiplos fatores, entre os quais estilos de vida e fatores ambientais, e apresentam consequências significativas na vida do adolescente e posteriormente na idade adulta. O sono e as suas perturbações deveriam constituir uma preocupação para os profissionais da saúde e da educação com o objetivo de tornar os hábitos de sono saudáveis num estilo de vida - com benefícios calculáveis como os associados a outros estilos de vida saudáveis (alimentação e exercício físico). Em Portugal, os estudos sobre problemas do sono em adolescentes são escassos, bem como as intervenções individuais e comunitárias no âmbito da higiene do sono. Os objetivos desta investigação foram estimar a prevalência de insónia e de sintomas de insónia em adolescentes, identificar fatores de risco e protetores dos sintomas de insónia, analisar as repercussões dos sintomas de insónia, caracterizar os padrões de sono dos adolescentes do distrito de Viseu e elaborar uma proposta de intervenção destinada à promoção da higiene do sono adaptada às características dos adolescentes do distrito de Viseu. Métodos: Realizou-se um estudo transversal onde se avaliaram alunos de vinte e seis escolas públicas do terceiro ciclo e secundário do distrito de Viseu, durante ano letivo 2011/2012. A recolha dos dados foi efetuada através de um questionário autoaplicado e respondido pelos alunos em sala de aula. Foram considerados elegíveis para participar no estudo todos os alunos que frequentassem entre o 7.º e o 12.º ano de escolaridade e tivessem idades entre os 12 e os 18 anos. Dos 9237 questionários distribuídos recolheu-se 7581 (82,1%). Foram excluídos da análise os questionários relativos a adolescentes com idade inferior a 12 ou superior a 18 anos e os questionários devolvidos por preencher. A amostra global foi constituída por 6919 adolescentes, sendo 3668 (53,2%) do sexo feminino. A insónia foi definida com base na presença, no mês prévio, dos sintomas de insónia definidos nos critérios do DSM-IV (dificuldade em adormecer, dificuldade em manter o sono, acordar muito cedo e ter dificuldade em voltar a adormecer e sono não reparador) com uma frequência de pelo menos três vezes por semana e associados a consequências no dia-a-dia. A qualidade de vida foi avaliada com recurso à escala de qualidade de vida SF-36; a sintomatologia depressiva através do Inventário de Depressão de Beck para adolescentes (BDI-II) e a sonolência diurna utilizando a Escala de Sonolência de Epworth (ESE). Para responder ao último objetivo foi elaborada uma proposta de intervenção individual e comunitária no âmbito da higiene do sono. A proposta resulta da evidência científica, dos resultados da presente investigação e de reuniões com profissionais da saúde e da educação. Resultados: No total da amostra, a prevalência de insónia foi de 8,3% e de sintomas de insónia foi de 21,4%. A prevalência de insónia foi superior no sexo feminino (10,1% vs. 5,9%; p12º ano OR ajustado= 0,64; IC95%: 0,47-0,87). Após uma análise multivariada, o modelo preditivo para a ocorrência de sintomas de insónia incluiu as variáveis sexo feminino, viver em meio urbano, consumir café e apresentar sintomatologia depressiva. Este modelo apresenta uma especificidade de 84,2% e uma sensibilidade de 63,6%. O sono insuficiente associou-se, após ajuste para o sexo e idade, com o ano de escolaridade, estado civil dos pais, determinados estilos de vida (consumo de café, tabagismo, consumo de álcool, consumo de outras drogas, sair à noite, presença de TV no quarto e número de horas despendido a ver televisão e no computador), latência do sono, sesta > 30 minutos, horários de sono irregulares e com a toma de medicamentos para dormir. Os resultados deste estudo constituem um diagnóstico de situação relativamente aos problemas de sono em adolescentes no distrito de Viseu. Tendo por base os princípios da Carta de Ottawa relativamente à promoção da saúde, a proposta elaborada visa a implementação de estratégias de prevenção agrupadas em intervenções individuais, comunitárias e sobre os planos curriculares. As intervenções baseiam-se na utilização das tecnologias da informação e comunicação, no contexto da nova arquitetura na esfera pública da saúde conducente aos sistemas personalizados de informação em saúde (SPIS). Conclusões: Registou-se uma elevada prevalência de insónia e sintomas de insónia entre os adolescentes do distrito de Viseu, superior no sexo feminino. A presença de sintomas de insónia esteve associada, sobretudo, a determinados estilos de vida e à ausência de higiene do sono. Os problemas de sono em adolescentes, devido à sua frequência e repercussões, devem constituir uma preocupação em termos de saúde pública e constituir uma prioridade nas estratégias de educação para a saúde. Os 9 princípios da intervenção delineada visam uma abordagem preventiva de problemas de sono - através da ação conjunta de profissionais da saúde e da educação, de elementos da comunidade e com o indispensável envolvimento dos adolescentes e da família -, procurando instituir os hábitos de sono saudáveis como um estilo de vida. ABSTRACT - Introduction: Sleep disorders, namely insomnia, insomnia symptoms, inadequate sleep patterns and daytime sleepiness, are frequent in adolescents. These problems are frequently associated to various factors, among which lifestyle and environmental factors, and present substantial consequences during adolescence and adulthood. Sleep and sleep disorders should be a constant concern for health and education professionals with the objective of building healthy sleep habits into a life style – with measurable benefits as those associated to other healthy life styles (diet and physical activity). In Portugal there is little investigation on sleep disorders among adolescents, as well as rare individual or community interventions on sleep hygiene. The objectives of this investigation were to estimate the prevalence of insomnia and insomnia symptoms among adolescents, identify risk and protective factors for insomnia symptoms, examine the repercussions of insomnia symptoms, characterize sleep patterns among adolescents from the district of Viseu and elaborate a proposal of intervention for the promotion of sleep hygiene adjusted to the characteristics of the adolescents of the district of Viseu. Methods: We carried out a cross-sectional study evaluating students from twenty-six public secondary schools from the district of Viseu during 2011-2012 academic year. Data gathering was done using a self-applied questionnaire answered by the students in the classroom. All students from the 7th to the 12th grade and aged 12 to 18 years were eligible. From 9237 questionnaires distributed we collected 7581 (82.1%). All unfilled questionnaires and those from students younger than 12 years old or older than 18 years old were excluded from analysis. The final sample was made of 6919 adolescents, of which 3668 (53.2%) were females. Insomnia was defined based on the presence, on the preceding month, of insomnia symptoms as defined by DSM-IV criteria (difficulty initiating sleep, difficulty maintaining sleep, early morning awakening and non-restoring sleep) at least three times per week and resulting in daily life consequences. Quality of life was evaluated with the quality of life health survey SF-36, depressive symptomatology with Beck’s Depression Inventory for adolescents (BDI-II) and daytime sleepiness with the Epworth Sleepiness Scale. To accomplish the final objective we elaborated a proposal for an individual and communitarian intervention regarding sleep hygiene. This proposal derives from scientific evidence, the results of our investigation and meetings with health and education professionals. Results: The prevalence of insomnia in the whole sample was 8.3% and the prevalence of insomnia symptoms was 21.4%. The prevalence of insomnia was superior in females (10.1% vs. 5,9%; p12th grade adjusted OR= 0.64; CI95%: 0.47-0.87). After multivariate analysis, the predictive model for the occurrence of insomnia symptoms included the variables female sex, urban residence, coffee consumption and presence of depressive symptoms. This model presents an 84.2% specificity and 63.6% sensitivity. For insufficient sleep, after adjustment for sex and age there was an association with school grade, parent’s marital status, certain lifestyles (coffee consumption, smoking, alcohol consumption, illicit drugs consumption, nightlife, TV in the bedroom and time spent watching TV or using a computer), sleep latency, naps > 30 minutes, irregular bedtime and taking medications to sleep. This study’s results establish a situational diagnosis of sleep disturbances among adolescents in the district of Viseu. Based on the principles of The Ottawa Charter for Health Promotion, a proposal was developed aiming to implement prevention strategies grouped in individual, communitarian and curricular programs interventions. Interventions are based on the use of information and communication technologies. As such, these interventions will participate in the architecture of public health leading to personally controlled health records (PCHR). Conclusions: A high prevalence of insomnia and insomnia symptoms was disclosed among adolescents in the district of Viseu. These prevalences were higher in the female sex. Insomnia symptoms were mainly associated with particular lifestyles and lack of sleep hygiene. Sleep disorders among adolescents, due to their frequency and deleterious effects, must represent a public health concern and a priority in education for health strategies. The objective of the 9 principles of the outlined intervention is to prevent sleep disorders – through the combined action of health and education professionals, community partners and the obligatory involvement of adolescents and families-, establishing healthy sleep routines as a lifestyle.

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    Authors: Marques, Eduardo Miguel da Silva;

    Após a proibição da utilização de dióxido de titânio (E171) no mercado Francês, muitas empresas por toda a Europa decidiram começar a substituir este aditivo nos seus produtos. Recentemente em outubro de 2020 o Parlamento Europeu levantou uma objeção contra a proposta de emenda à especificação e utilização de E171, que foi aceite. Foi pedido à Comissão que aplique o princípio de precaução e que remova o E171 da lista de aditivos alimentares permitidos na União Europeia. Estes desenvolvimentos podem resultar na proibição de E171 num futuro próximo, que teria um impacto enorme dada a sua utilização em diversas aplicações. Este trabalho pretendeu testar diferentes estratégias de substituição de E171 em seis pré-preparados de creme de pasteleiro na Empresa X. As estratégias testadas foram misturas lácteas, corantes, e misturas emulsionantes. A substituição mais viável foi com emulsionante ME2 e foi submetida a avaliação de características organoléticas, colorimétrica, de estabilidade (calor e sinérese), de aplicação, de qualidade (pH e textura) e de custo financeiro. Os resultados indicaram que o emulsionante ME2 apresenta uma capacidade opacificante semelhante a E171 numa substituição de 1:5, no entanto resultam diferenças reológicas que necessitam ser avaliadas com maior detalhe. Este trabalho indica que neste tipo de produtos é possível substituir E171 utilizando emulsionantes.

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    Authors: Costa, Ana Rita Neves;

    O jornalismo cultural acompanhou o processo de evolução da sociedade e dos meios de comunicação social. Dos ensaios literários às hard news, as mudanças ocorridas nas secções, editorias e publicações especializadas em Cultura são visíveis e quase palpáveis. O jornalismo cultural, outrora conhecido pelo caráter refletivo e crítico, entrou na espiral da indústria e da velocidade da informação. A agenda cultural ditada por inúmeras fontes de informação, empresas e agências de comunicação tornou-se indispensável no quotidiano das redações e permanece hoje, como denominador comum de muitos conteúdos. Com base num estágio curricular realizado no jornal online Observador durante três meses, na secção de Cultura, tenta-se perceber de que forma esta problemática molda o trabalho jornalístico contemporâneo. Cultural journalism went along with the evolution’s process of the society and the media. From literary essays to hard news, the transformations occured in Culture’s sections, editorials and specialized publications are visible and almost touchable. Cultural Journalism, once known by the reflective and critical character, went in a spiral of the industry and the speed up information. The cultural agenda dictated by several sources, companies and communication agencies, became mandatory in the everyday of the newsrooms; and today, it is the common denominator of many content. Based on the curricular intership accomplished on the online newspaper Observador for three months, in the Culture section, one tries to understand in which way is this issue shaping journalistic work nowadays

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