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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Formisano, Annarita; Hunsberger, Monica; Bammann, Karin; Vanaelst, Barbara; +7 Authors

    AbstractObjectiveTo analyse the association between family structure and adiposity in children.DesignCross-sectional and longitudinal analysis of the IDEFICS (Identification and prevention of dietary- and lifestyle-induced health effects in children and infants) study cohort.SettingPrimary schools and kindergartens.SubjectsChildren (n12 350; aged 7·9 (sd1·8) years) for the cross-sectional analysis and children (n5236; at baseline: normal weight, aged 5·9 (sd1·8) years) for the longitudinal study underwent anthropometry. Family structure was analysed as (i) number and type of cohabiting adults and (ii) number of siblings.ResultsIn the cross-sectional analysis, after controlling for covariates, children living with grandparents had significantly higher BMIZ-score than those living with both parents (0·63; 95 % CI 0·33, 0·92v. 0·19; 95 % CI 0·17, 0·22;P< 0·01); in addition, the higher the number of siblings, the lower the BMIZ-score (only child = 0·31; 95 % CI 0·24, 0·38; 1 sibling = 0·19; 95 % CI 0·16, 0·23; 2 siblings = 0·15; 95 % CI 0·09, 0·20; >2 siblings = 0·07, 95 % CI 0·04, 0·19;P< 0·001). Over the 2-year follow-up, differences in weight gain were observed across family-structure categories. Further, the risk of incidence of overweight/obesity was significantly lower the higher the number of siblings living in the household (v. only child: 1 sibling = 0·74, 95 % CI 0·57, 0·96; 2 siblings = 0·63, 95 % CI 0·45, 0·88; >2 siblings = 0·40, 95 % CI 0·21, 0·77), independently of confounders.ConclusionsThe study suggests that an independent association between family structure and childhood obesity exists.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Public Health Nutrit...arrow_drop_down
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    Public Health Nutrition
    Article . 2013 . Peer-reviewed
    License: Cambridge Core User Agreement
    Data sources: Crossref
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Europe PubMed Central
    Other literature type . 2013
    Data sources: PubMed Central
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    CNR ExploRA
    Article . 2013
    Data sources: CNR ExploRA
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Public Health Nutrit...arrow_drop_down
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      Public Health Nutrition
      Article . 2013 . Peer-reviewed
      License: Cambridge Core User Agreement
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      Europe PubMed Central
      Other literature type . 2013
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      CNR ExploRA
      Article . 2013
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: V. Sreekanth; Cathryn Tonne; Maëlle Salmon; S. Arulselvan; +1 Authors

    We describe how reference filter characteristics (unexposed weights) can influence instrument-reported attenuation (thereby black carbon concentrations) using the OT21 (SootScan Optical Transmissometer), in the case of Emfab filters. Reference filters (unexposed filters) with higher unexposed weights (pre-weights) compared to that of sample filters are found to reduce the instrument-reported aerosol sample attenuation measurement; reference filters weighing less than the sample filter results in the reverse outcome. This sensitivity can lead to significant under- and over-estimation by the instrument (estimated mean absolute error: ∼ 10 and 16 ATN units for infrared (IR) and ultraviolet (UV) channels, respectively, in our tests, which represent 44% and 15% errors, respectively). We find that the error is linear with respect to difference in the unexposed filter weights of the sample and reference filters. We report two equations (one for IR, one for UV) for correcting the Transmissometer output based on experimental data, which allow use of any unexposed Emfab filter as the reference filter; application of correction parameters to data here reduces the error to 2 and 3 ATN units, respectively, for IR and UV (10% and 3% errors, respectively). The research leading to these results received funding from the European Research Council under ERC Grant Agreement number 336167 for the CHAI Project. We are thankful to field staff of CHAI project and Sri Ramachandra University, Chennai for their collaboration throughout the monitoring and measurements. Filter weight data is available from the corresponding author on request.

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    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Journal of Aerosol Science
    Article . 2019 . Peer-reviewed
    License: Elsevier TDM
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ UPF Digital Reposito...arrow_drop_down
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Journal of Aerosol Science
      Article . 2019 . Peer-reviewed
      License: Elsevier TDM
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Della Pia, A.; Luo, D.; Blackwell, R.; Costantini, G.; +1 Authors

    Self-assembly of three related molecules – terephthalic acid and its hydroxylated analogues – at liquid/solid interfaces (graphite/heptanoic acid and graphite/1-phenyloctane) has been studied using a combination of scanning tunnelling microscopy and molecular mechanics and molecular dynamics calculations. Brickwork-like patterns typical for terephthalic acid self-assembly have been observed for all three molecules. However, several differences became apparent: (i) formation or lack of adsorbed monolayers (self-assembled monolayers formed in all systems, with one notable exception of terephthalic acid at the graphite/1-phenyloctane interface where no adsorption was observed), (ii) the size of adsorbate islands (large islands at the interface with heptanoic acid and smaller ones at the interface with 1-phenyloctane), and (iii) polymorphism of the hydroxylated terephthalic acids’ monolayers, dependent on the molecular structure and/or solvent. To rationalise this behaviour, molecular mechanics and molecular dynamics calculations have been performed, to analyse the three key aspects of the energetics of self-assembly: intermolecular, substrate–adsorbate and solvent–solute interactions. These energetic characteristics of self-assembly were brought together in a Born–Haber cycle, to obtain the overall energy effects of formation of self-assembled monolayers at these liquid/solid interfaces.\ud \ud

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ CORE (RIOXX-UK Aggre...arrow_drop_down
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Fernando T. Maestre; Ricard V. Solé; Brajesh K. Singh;

    We briefly review how microbial biotechnology can contribute to improve activities aiming to restore degraded drylands and to combat their desertification, which are an integral part of the Sustainable Development Goal 15 of the 2030 Agenda. Microbial biotechnology offers notable promise to improve restoration actions based on the use of biocrust-forming engineered cyanobacteria, which play key roles in maintaining ecosystem structure and functioning in drylands worldwide. Advances in our understanding of microbiome associated to biocrusts and of the signalling involved in the communication among their constituents can also potentially enhance the outcome of restoration activities in drylands. FTM acknowledges support from the European Research Council (ERC GA 647038 [BIODESERT]) and from the Alexander von Humboldt Stiftung (Humboldt Research Award) during the writing of this manuscript. RS has been supported by an ERC Advanced Grant GA 294294 (SYNCOM), by the Botín Foundation by Banco Santander through its Santander Universities Global Division, a MINECO FIS2015-6761 fellowship and by the Santa Fe Institute. The work of BKS on this topic is funded by the Australian Research Council.

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    Microbial Biotechnology
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      Microbial Biotechnology
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    Authors: H. Goelzer; H. Goelzer; H. Goelzer; S. Nowicki; +49 Authors

    Abstract. The Greenland ice sheet is one of the largest contributors to global meansea-level rise today and is expected to continue to lose mass as the Arcticcontinues to warm. The two predominant mass loss mechanisms are increasedsurface meltwater run-off and mass loss associated with the retreat ofmarine-terminating outlet glaciers. In this paper we use a large ensemble ofGreenland ice sheet models forced by output from a representative subset ofthe Coupled Model Intercomparison Project (CMIP5) global climate models to project ice sheet changes and sea-level risecontributions over the 21st century. The simulations are part of theIce Sheet Model Intercomparison Project for CMIP6 (ISMIP6). We estimate thesea-level contribution together with uncertainties due to future climateforcing, ice sheet model formulations and ocean forcing for the twogreenhouse gas concentration scenarios RCP8.5 and RCP2.6. The resultsindicate that the Greenland ice sheet will continue to lose mass in bothscenarios until 2100, with contributions of 90±50 and 32±17 mm to sea-level rise for RCP8.5 and RCP2.6, respectively. The largestmass loss is expected from the south-west of Greenland, which is governed bysurface mass balance changes, continuing what is already observed today.Because the contributions are calculated against an unforced controlexperiment, these numbers do not include any committed mass loss, i.e. massloss that would occur over the coming century if the climate forcingremained constant. Under RCP8.5 forcing, ice sheet model uncertaintyexplains an ensemble spread of 40 mm, while climate model uncertainty andocean forcing uncertainty account for a spread of 36 and 19 mm,respectively. Apart from those formally derived uncertainty ranges, thelargest gap in our knowledge is about the physical understanding andimplementation of the calving process, i.e. the interaction of the ice sheetwith the ocean. info:eu-repo/semantics/published

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    Authors: Majid Zamiri; Andreia Artifice; Elsa Marcelino-Jesus; Joao Sarraipa; +1 Authors

    The widespread use of sensorial technologies has created new opportunities for enterprises, as these new sources of data assist in increasing the context-awareness in which enterprises operate and enable them to better anticipate and adapt to changes in the business environment. This leads to better decision-making and greater profitability, as well as to reduced operating risks. However, if enterprises are to get any benefit from these new data sources, there is a need for tools, which will enable them to deal with the complexity of the multitude of data sources, both from physical and virtual objects, as well as a means to extract relevant and correct information and knowledge from it. Having access to data is not enough. The real value to enterprises comes from being able to process it, interpret it and being able to make accurate forecasts upon which they can base their business decisions. This paper presents the blockchain technology which is intended to support the development of sensing enterprise systems for intelligent knowledge management. Since the creation of the internet, blockchain is the most important technology created that is in constant development and has still much more to develop. In a simple way, blockchain is a computational technology for register of operations, decentralized, free-access, transparent, global, continuous. It is a public database that is accessible for everyone and is much more secure and reliable than other forms currently known to perform similar operations. Thus, it is intended to demonstrate the importance of this recent technology in business processes and the future trends of its use in sensing enterprise business processes. The proposed framework intends to demonstrate and serve as the foundation for new business models supported by the new capabilities provided by sensorial technologies in the support of enterprise applications.

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    Authors: Eisenhofer, Graeme; Peitzsch, Mirko; McWhinney, Brett C.;

    AbstractApplications of LC-MS/MS for the biochemical diagnosis of catecholamine-producing tumors have followed from parallel emergence of this technology with changes in emphasis of biochemical testing, in particular requirements that testing include measurements of plasma or urinary fractionated metanephrines. Since the turn of the century, when LC-MS/MS was first introduced into the routine laboratory, there have been numerous advances in analytical methodology that offer opportunities for breaking away from out-moded analytical procedures to methods that provide enhanced diagnostic information with dramatically improved analytical sensitivity, accuracy and precision as well as rapid sample throughput. LC-MS/MS also offers high analytical specificity, but is not infallible. Multiple reaction monitoring does not always guarantee selectivity and attention to sample purification and chromatographic separation remains important. Although other analytical methods persist, today LC-MS/MS represents the method of choice for high-throughput, low-cost, precise and accurate measurements of the catecholamine metabolites now routinely used for diagnosis of catecholamine-producing tumors.

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    Authors: Meinshausen, Maren; Rieckert, Anja; Renom-Guiteras, Anna; Kröger, Moritz; +5 Authors

    Search string search 1 and 2. (DOCX 102 kb)

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    Authors: De Simone, Mario; Alvigini, Laura; Alonso-Cotchico, Lur; Brissos, Vânia; +6 Authors

    Funding Information: This project has received funding from the Biobased Industries Joint Undertaking (JU) under Grant Agreement 837890 (SMARTBOX) and from Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) Grant 824017 (B-Ligzymes). The JU receives support from the European Union’s Horizon 2020 research and innovation program and the Biobased Industries Consortium (BIC). Fundação para a Ciência e a Tecnologia (FCT) additionally supported this work through R&D Unit MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020), LS4FUTURE Associated Laboratory (LA/P/0087/2020), and Project UID/Multi/04326/2019, from the operational programs CRESC Algarve 2020 and COMPETE for 2020 through Project EMBRC.PT ALG-01-0145-FEDER-022121. M.D.S. acknowledges a Ph.D. grant (2020.08246.BD) from FCT. Publisher Copyright: © 2022 American Chemical Society. Biocatalysis is a key tool in both green chemistry and biorefinery fields. NOV1 is a dioxygenase that catalyzes the one-step, coenzyme-free oxidation of isoeugenol into vanillin and holds enormous biotechnological potential for the complete valorization of lignin as a sustainable starting material for biobased chemicals, polymers, and materials. This study integrates computational, kinetic, structural, and biophysical approaches to characterize a new NOV1 variant featuring improved activity and stability compared to those of the wild type. The S283F replacement results in a 2-fold increased turnover rate (kcat) for isoeugenol and a 4-fold higher catalytic efficiency (kcat/Km) for molecular oxygen compared to those of the wild type. Furthermore, the variant exhibits a half-life that is 20-fold higher than that of the wild type, which most likely relates to the enhanced stabilization of the iron cofactor in the active site. Molecular dynamics supports this view, revealing that the S283F replacement decreases the optimal pKa and favors conformations of the iron-coordinating histidines compatible with an increased level of binding to iron. Importantly, whole cells containing the S283F variant catalyze the conversion of ≤100 mM isoeugenol to vanillin, yielding >99% molar conversion yields within 24 h. This integrative strategy provided a new enzyme for biotechnological applications and mechanistic insights that will facilitate the future design of robust and efficient biocatalysts. publishersversion published

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    Biochemistry
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    Biochemistry
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      Biochemistry
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      Biochemistry
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    Authors: Praher, Daniela; Zimmermann, Bob; Dnyansagar, Rohit; Miller, David J.; +10 Authors

    S6_Spi_output.pdf

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    Authors: Niclas G. Karlsson; Chunsheng Jin; Miguel A. Rojas-Macias; Barbara Adamczyk;
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    Trends in Glycoscience and Glycotechnology
    Article . 2017 . Peer-reviewed
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      Trends in Glycoscience and Glycotechnology
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    Authors: Yizhou Yang; Martin Ratsch; Austin M. Evans; Karl Börjesson;

    The development of covalent organic frameworks (COFs) during the past decades has led to a variety of promising applications within gas storage, catalysis, drug delivery, and sensing. Even though most described synthesis methods result in powdery COFs with uncontrolled grain size, several approaches to grow COF films have recently been explored. However, in all COFs so far presented, the isolated materials are chemically homogeneous, with all functionalities homogeneously distributed throughout the entire material. Strategies to synthetically manipulate the spatial distribution of functionalities in a single film would be game changing. Specifically, this would allow for the introduction of local functionalities and even consecutive functions in single frameworks, thus broadening their synthetic versatility and application potential. Here, we synthesize two 3D crystalline COF films. The frameworks, the ionic B-based and neutral C-based COFs, have similar unit cell parameters, which enables their epitaxial stacking in a layered 3D COF film. The film growth was monitored in real time using a quartz crystal microbalance, showing linear growth with respect to reaction time. The high degree of polymerization was confirmed by chemical analysis and vibrational spectroscopy. Their polycrystalline and anisotropic natures were confirmed with grazing incidence X-ray diffraction. We further expand the scope of the concept by making layered films from COF-300 and its iodinated derivative. Finally, the work presented here will pave the path for multifunctional COF films where concurrent functionalities are embedded in the same crystalline material.

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    Journal of the American Chemical Society
    Article . 2023 . Peer-reviewed
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